Difference between revisions of "Part:BBa K541545:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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'''THE SUICIDE EXPERIMENT OF E. COLI
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how you used this part and how it worked out.
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Our K541545 part contains IPTG promoter and the gene part of LALF protein on a backbone which has resistance to chloramphenicol. We transported this part to E. coli for testing whether E. coli would kill itself or not.
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Experiments of plates with and without IPTG
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Plate A1: We streaked E coli culture which has K541545 on this plate and the plate includes IPTG and Chloramphenicol.
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Plate A2: We streaked E coli culture which has K541545 on this plate and the plate includes just Chloramphenicol.
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Plate B1: This plate does not have IPTG and we streaked E.coli culture which does not have IPTG promoter.
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PlateB2: This plate has IPTG and we streaked E coli culture which has IPTG promoter.
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We prepared the B1 and B2 plates for testing toxic effect of IPTG. Because we wanted to be sure that the only factor affecting growth of E coli is LALF protein.
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[[Image:rar1.jpg]]
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[[Image:rar2.jpg]]
  
 
===Applications of BBa_K541545===
 
===Applications of BBa_K541545===

Revision as of 02:55, 22 September 2011

THE SUICIDE EXPERIMENT OF E. COLI Our K541545 part contains IPTG promoter and the gene part of LALF protein on a backbone which has resistance to chloramphenicol. We transported this part to E. coli for testing whether E. coli would kill itself or not.

Experiments of plates with and without IPTG

Plate A1: We streaked E coli culture which has K541545 on this plate and the plate includes IPTG and Chloramphenicol.

Plate A2: We streaked E coli culture which has K541545 on this plate and the plate includes just Chloramphenicol.

Plate B1: This plate does not have IPTG and we streaked E.coli culture which does not have IPTG promoter.

PlateB2: This plate has IPTG and we streaked E coli culture which has IPTG promoter.

We prepared the B1 and B2 plates for testing toxic effect of IPTG. Because we wanted to be sure that the only factor affecting growth of E coli is LALF protein.

File:Rar1.jpg

File:Rar2.jpg

Applications of BBa_K541545

User Reviews

UNIQ39919d8ffefc9f63-partinfo-00000000-QINU UNIQ39919d8ffefc9f63-partinfo-00000001-QINU