Difference between revisions of "Part:BBa K515004"
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| − | This sequence has been taken from BBa_K112808 (Berkeley 2008's kill switch cassette) and has been modified through the addition of an insulator sequence between our newly created RBS and the coding sequence. The insulator sequence has been specially developed to allow the easy switching of our parts between promoters while maintaining little homology with the vector making recombination events between the insulator sequence and the vector unlikely. We have also added a double TAA terminator sequence instead of the original single TGA sequence. | + | This sequence has been taken from BBa_K112808 (Berkeley 2008's kill switch cassette) and has been modified through the addition of an insulator sequence between our newly created RBS and the coding sequence. The insulator sequence has been specially developed to allow the easy switching of our parts between promoters while maintaining little homology with the vector making recombination events between the insulator sequence and the vector unlikely. We have also added a double TAA terminator sequence instead of the original single TGA sequence.</p> |
| + | <p>For more information visit the composite construct <a href="https://parts.igem.org/Part:BBa_K515104"/>BBa_K515104</a>. | ||
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Revision as of 02:20, 22 September 2011
T4 Anti-Holin
This sequence has been taken from BBa_K112808 (Berkeley 2008's kill switch cassette) and has been modified through the addition of an insulator sequence between our newly created RBS and the coding sequence. The insulator sequence has been specially developed to allow the easy switching of our parts between promoters while maintaining little homology with the vector making recombination events between the insulator sequence and the vector unlikely. We have also added a double TAA terminator sequence instead of the original single TGA sequence.
For more information visit the composite construct BBa_K515104.
Sequence and Features