Difference between revisions of "Part:BBa K515104"
Line 21: | Line 21: | ||
<!-- --> | <!-- --> | ||
<html> | <html> | ||
− | <p | + | <p>This BioBrick has been sequence verified. It has also been tested for protein expression. A nice clear band of the appropriate size can be seen at the bottom of the gel indicating a strong over-expression of a small protein of the appropriate size.</p> |
<div class="imgbox" style="width:300px;margin:0 auto;"> | <div class="imgbox" style="width:300px;margin:0 auto;"> |
Revision as of 02:19, 22 September 2011
J23100 promoter - Antiholin
A composite part between J23100 and anti-holin (BBa_K515004). The promoter has been chosen in order to comply with our Gene Guard device specifications. It is under the influence of a strong promoter due to the lower copy number of this gene (will be integrated into the genome) in comparison to that of the Holin gene which will be in a high copy number plasmid within our system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 276
This BioBrick has been sequence verified. It has also been tested for protein expression. A nice clear band of the appropriate size can be seen at the bottom of the gel indicating a strong over-expression of a small protein of the appropriate size.
Figure 1: Protein gel showing an over-expression band (higher in intensity) of a small protein. Lane 1 and 2 are control and lane 3 and 4 are the anti-holin expressing cells.(Data by Imperial College London iGEM team 2011).