Difference between revisions of "Part:BBa K515007"
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[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.
 
[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.
 
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K515007 SequenceAndFeatures</partinfo>
 
  
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===Functional Parameters===
 
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<partinfo>BBa_K515007 parameters</partinfo>
 
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<p><b>This BioBrick has been sequence verified.</p></b>
 
<p><b>This BioBrick has been sequence verified.</p></b>

Revision as of 01:46, 22 September 2011

Dendra2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

This part consists of coding sequence codon optimised for Escherichia coli. It is part of the composite biobrick BBa_K515107. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm[1].

Reference

[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.

This BioBrick has been sequence verified.