Difference between revisions of "Part:BBa K515007"
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K515007 short</partinfo>
 
<partinfo>BBa_K515007 short</partinfo>
 +
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K515007 SequenceAndFeatures</partinfo>
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K515007 parameters</partinfo>
 +
<!-- -->
 +
  
 
<html>
 
<html>

Revision as of 01:46, 22 September 2011

Dendra2


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

This part consists of coding sequence codon optimised for Escherichia coli. It is part of the composite biobrick BBa_K515107. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm[1].

Reference

[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.