Difference between revisions of "Part:BBa K515007"
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<p><b>Description</b></p>
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<h2>Description</h2>
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<p>This part consists of coding sequence codon optimised for <i>Escherichia coli</i> & <i>Bacillus subtilis</i>. It is part of the composite biobrick <a href="https://parts.igem.org/Part:BBa_K515107">BBa_K515107</a>. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm (Gurskaya et al., 2006).</p>
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<p>This part consists of coding sequence codon optimised for <i>Escherichia coli</i>. It is part of the composite biobrick <a href="https://parts.igem.org/Part:BBa_K515107">BBa_K515107</a>. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm<sup>[1]</sup>.</p>
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<h2>Reference</h2>
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[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465.
 
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Revision as of 01:43, 22 September 2011

Dendra2

Description

This part consists of coding sequence codon optimised for Escherichia coli. It is part of the composite biobrick BBa_K515107. This sequence codes for photoconvertible protein Dendra, capable of being irreversibly photoconverted by single-photon stimulation from excitation at 486nm and emission at 505nm wavelength to 558nm excitation and 575nm emission wavelength. Photoconversion can occur using two wavelengths 488 and 405nm[1].

Reference

[1] Gurskaya N et al. (2006) Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology 24: 461-465. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This BioBrick has been sequence verified.