Difference between revisions of "Part:BBa K676011:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 10: Line 10:
 
Entire pSC101 Sequence - 9263 bp
 
Entire pSC101 Sequence - 9263 bp
  
−
CAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAATCAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCAGACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTATGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTACCCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAAGACAGCGACTCAGGTGCCTGATGGTCGGAG
+
CAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAAT
 +
CAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCA
 +
GACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTA
 +
TGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTAC
 +
CCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAA
 +
GACAGCGACTCAGGTGCCTGATGGTCGGAG
  
 
===Source===
 
===Source===

Revision as of 01:36, 22 September 2011

Gyrase Binding Site from pSC101


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR and then 2 parts ligation The GBS in pSC101 plasmid (285 bp) from 4580 to 4864: Entire pSC101 Sequence - 9263 bp

CAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAAT CAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCA GACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTA TGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTAC CCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAA GACAGCGACTCAGGTGCCTGATGGTCGGAG

Source

pSC101 plasmid

References