Difference between revisions of "Part:BBa K676011:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K676011 short</partinfo> | <partinfo>BBa_K676011 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
PCR and then 2 parts ligation | PCR and then 2 parts ligation | ||
| + | The GBS in pSC101 plasmid (285 bp) from 4580 to 4864: | ||
| + | Entire pSC101 Sequence - 9263 bp | ||
| − | + | CAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAATCAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCAGACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTATGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTACCCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAAGACAGCGACTCAGGTGCCTGATGGTCGGAG | |
===Source=== | ===Source=== | ||
Revision as of 01:35, 22 September 2011
Gyrase Binding Site from pSC101
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR and then 2 parts ligation The GBS in pSC101 plasmid (285 bp) from 4580 to 4864: Entire pSC101 Sequence - 9263 bp
CAGTCTGAATGACCTGTCACGGGATAATCCGAAGTGGTCAGACTGGAAAATCAGAGGGCAGGAACTGCTGAACAGCAAAAAGTCAGATAGCACCACATAGCAGACCCGCCATAAAACGCCCTGAGAAGCCCGTGACGGGCTTTTCTTGTATTATGGGTAGTTTCCTTGCATGAATCCATAAAAGGCGCCTGTAGTGCCATTTACCCCCATTCACTGCCAGAGCCGTGAGCGCAGCGAACTGAATGTCACGAAAAAGACAGCGACTCAGGTGCCTGATGGTCGGAG
Source
pSC101 plasmid