Difference between revisions of "Part:BBa E1010"

Revision as of 01:30, 22 September 2011

**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)

monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm

Usage and Biology

Robert E. Campbell started with Discosoma RFP (DsRed) and evolved a faster folding, monomeric variant. See paper listed in source. Codon optimized for expression in bacteria (?? DE)

iGEM11_Uppsala-Sweden: Expression of chromoproteins. The images above show E coli constitutively expressing amilCP BBa_K592009 (blue), amilGFP BBa_K592010 (yellow) and RFP BBa_E1010 (red).

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 555
Illegal AgeI site found at 667
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_E1010 short</partinfo>
@@ Line 7: / Line 6: @@
 Excitation peak: 584 nm
 Emission peak: 607 nm
 ===Usage and Biology===
 Robert E. Campbell started with Discosoma RFP (DsRed) and evolved a faster folding, monomeric variant.  See paper listed in source.  Codon optimized for expression in bacteria (?? DE)
+[[Image:AmilCP_amilGFP_RFP.jpg|300px]]
+[[Image:On cultures BYR small.jpg|300px]]
+[[Image:Pellets BYR.jpg|300px]]
+'''iGEM11_Uppsala-Sweden:''' Expression of chromoproteins. The images above show ''E coli'' constitutively expressing amilCP <partinfo>BBa_K592009</partinfo> (blue), amilGFP <partinfo>BBa_K592010</partinfo> (yellow) and RFP <partinfo>BBa_E1010</partinfo> (red).
 <span class='h3bb'>Sequence and Features</span>