Difference between revisions of "Part:BBa K613017:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The mutation was introduced by site-directed mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007].
+
The mutation was introduced by PCR-induced mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007].
  
 
===Source===
 
===Source===

Latest revision as of 01:25, 22 September 2011

TetR Y42F mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutation was introduced by PCR-induced mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0378111907004623 Krueger et al, 2007].

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the Y42F mutation by site-directed mutagenesis. This mutation had already been described by Krueger et al (2007).

References