Difference between revisions of "Part:BBa I757010:Experience"

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This beta lactamase was used in combination with the TorA signal sequence (BBa_K208005) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.<br>
 
This beta lactamase was used in combination with the TorA signal sequence (BBa_K208005) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.<br>
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When <i>E.coli XL1 blue</i> where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C, or up to 300 µg/ml ampicillin, when incubated over night at 30 °C.<br>
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When <i>E.coli XL1 blue</i> where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C or at 30 °C.<br>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 01:07, 22 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I757010

Done by iGEM11 Potsdam_Bioware

This beta lactamase was used in combination with the TorA signal sequence (BBa_K208005) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.
When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C or at 30 °C.

User Reviews

UNIQcfeb822c9fe57f3f-partinfo-00000000-QINU UNIQcfeb822c9fe57f3f-partinfo-00000001-QINU The lactamases shows great activity at 37°C and 30°C incubation over night on agar plates. The transformed E.coli cells were able to survive with ampicillin concentrations up to 400 µg/ml at 37°C and at 30°C.