Difference between revisions of "Part:BBa K608102"

Revision as of 00:57, 22 September 2011

CcaS, green light receptor

CcaS is a cyanobacteriochrome, which is known to chromatically regulate the expression of the phycobilisome linker gene (cpcG2) in Synechocystis (see references). It functions as the receptor for green light and is made up of a N-terminal transmembrane helix, a cyanobactreria specific GAF domain, two PAS domains and a C-terminal histidine kinase. To be fully functional CcaS has to bind the chromophore Phycocyanobilin (PCB) with its GAF-domain. The GAF domain in this system has the ligation motif Cys-Leu, instead of the usual plant GAF-domain with Cys-His

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1685
Illegal SpeI site found at 1780
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1685
Illegal NheI site found at 1056
Illegal SpeI site found at 1780
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1685
Illegal XhoI site found at 6
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1685
Illegal SpeI site found at 1780
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1685
Illegal SpeI site found at 1780
Illegal AgeI site found at 1720
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K608102 short</partinfo>
 CcaS is a cyanobacteriochrome, which is known to chromatically regulate the expression of the phycobilisome linker gene (cpcG2) in Synechocystis (see references).
+It functions as the receptor for green light and is made up of a N-terminal transmembrane helix, a cyanobactreria specific GAF domain, two PAS domains and a C-terminal histidine kinase. To be fully functional CcaS has to bind the chromophore Phycocyanobilin (PCB) with its GAF-domain. The GAF domain in this system has the ligation motif Cys-Leu, instead of the usual plant GAF-domain with Cys-His