Difference between revisions of "Part:BBa K525998"
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T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from ''Escherichia coli'' but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, ''E. coli'' carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX). | T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from ''Escherichia coli'' but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, ''E. coli'' carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX). | ||
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| + | Used to express e.g. <partinfo>K525123</partinfo> - look there for typical induction profiles. | ||
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Revision as of 00:53, 22 September 2011
Promoter T7 and RBS
T7 promoter and RBS. The T7 promoter does not work with the RNA polymerase from Escherichia coli but with the RNA polymerase from the T7 phage. To express BioBricks under the control of a T7 promoter, E. coli carrying a T7 polymerase gene have to be used (e.g. BL21(DE3) or KRX).
Used to express e.g. BBa_K525123 - look there for typical induction profiles.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]