Difference between revisions of "Part:BBa K607019"
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This part is a member of [https://parts.igem.org/Lambda_cI_autoinducing_loop_family Lambda cI autoinducing loop family]. | This part is a member of [https://parts.igem.org/Lambda_cI_autoinducing_loop_family Lambda cI autoinducing loop family]. | ||
Revision as of 23:42, 21 September 2011
Prm_cI_antiPlasB with 0.07RBS
This part is a member of Lambda cI autoinducing loop family.
cI without degradation tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1061
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]