Difference between revisions of "Part:BBa K528021"

Latest revision as of 23:25, 21 September 2011

Expression Adapter fuse with E0030 YFP protein

Construct design

This device was used to measure expression level obtained from BBa_K528018 fused with Yellow Fluorescent Protein BBa_E0030. We have measured fluorescence level and compared it to BBa_K528010 B0034+YFP reference construct.

We have obtained the following results:

Part Name	Description	Expression level as %B0034+YFP	Std Dev.
BBa_K528021	BBa_K528018+YFP measurement device	20,37%	3,21%
reference	Bba_J23102+B0034+YFP (100% reference construct)	100,00%	2,61%

According to design BBa_K528018 has strength 22,86% relative to BBa_B0034 with given protein. This means that our expression adaptor works according to the design.

Authors

Clonning:

Paweł Urbański
Elżbieta Jankowska
Krzysztof Szczepaniak
Dorota Kaja Sabat

The lab work was supervised by:

Michał Lower
Ania Olchowik

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 8
Illegal NheI site found at 31
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 ===Construct design===
-To make truly standardize strength of RBS parts we propose a brand new part in synthetic biology - we call expression adapters. They are short sequences consisting of RBS, 5 bp spacer and a short (10 amino acid long) beginning of a protein.
+This device was used to measure expression level obtained from <partinfo>K528018</partinfo> fused with Yellow Fluorescent Protein <partinfo>E0030</partinfo>.
+We have measured fluorescence level and compared it to <partinfo>K528010</partinfo> B0034+YFP reference construct.
-For more info please look at Team Warsaw 2011 http://2011.igem.org/Team:Warsaw/ExpressionAdaptors/Results
+We have obtained the following results:
+<html><style type="text/css">
+table.tableizer-table {border: 1px solid #CCC; font-family: Arial, Helvetica, sans-serif; font-size: 12px;} .tableizer-table td {padding: 4px; margin: 3px; border: 1px solid #ccc;}
+.tableizer-table th {background-color: #104E8B; color: #FFF; font-weight: bold;}
+</style>
+<table class="tableizer-table">
+<tr class="tableizer-firstrow"><th>Part Name</th><th>Description</th><th>Expression level as %B0034+YFP</th><th>Std Dev.</th></tr> <tr><td>BBa_K528021</td><td>BBa_K528018+YFP measurement device</td><td>20,37%</td><td>3,21%</td></tr> <tr><td>reference</td><td>Bba_J23102+B0034+YFP (100% reference construct)</td><td>100,00%</td><td>2,61%</td></tr></table></html>
+According to design <partinfo>K528018</partinfo> has strength 22,86% relative to BBa_B0034 with given protein.
+This means that our expression adaptor works according to the design.
 ===Authors===