Difference between revisions of "Part:BBa K638401"
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===Usage and Biology===
 
===Usage and Biology===
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====Safety====
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The protein coding sequence for reflectin originally came from cells of an edible squid. There have been no reported safety issues for reflectins, so we do not anticipate the need for extra precautions when using this BioBrick part. See our [http://2011.igem.org/Team:Cambridge/Safety safety page] for more information.
  
 
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Revision as of 23:23, 21 September 2011

Arabinose inducible TorA Export Tagged Reflectin A1 Poly His generator

This construct is a Reflectin A1 protein with a TorA signal peptide fused to the N terminus, which has been shown to allow export of GFP via the twin arginine pathway (see information on Part:BBa_K638402). In addition a poly his tag has been added to the C terminus and a pBAD promoter will control expression of the fusion protein on induction with arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1369
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961