Difference between revisions of "Part:BBa K091104:Experience"
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'''Characterisation''' | '''Characterisation''' | ||
Revision as of 22:54, 21 September 2011
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K091104
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[http://2011.igem.org/Team:KULeuven K.U.Leuven iGEM 2011 Team] |
Characterisation We transformed Bba_K091104 (=pLac/Mnt hybrid) in DH5α-cells, followed by a minipreparation. After this we did a restriction digest with EcoRI and SpeI. Since the pSB1A2 vector is 2079bp and the Lac/Mnt hybrid promoter should be 87bp, we also expect a fragment of 87bp, one of 2079 and one of 2166bp. However, we see a fragment of 1000bp and of ±2000bp. |
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