Difference between revisions of "Part:BBa K676013"

Line 10: Line 10:
 
The higher level of expression can be use in making a more sensitive SPY device in order to detect shear stress level of the cells during fermentation as well as in downstream processing.
 
The higher level of expression can be use in making a more sensitive SPY device in order to detect shear stress level of the cells during fermentation as well as in downstream processing.
  
As with the BBa_K676001( Mu GBS ) , we also observed a slightly higher supercoiling efficiency ( about 10% higher compare to the controls) and better uniformity( smaller range of supercoiled topoisomers) in the supercoiled plasmids ( Mu GBS with SPY device) .   
+
As with the BBa_K676001( Mu GBS ) , we also observed a slightly higher supercoiling efficiency ( about 10% higher compare to the controls) and better uniformity (smaller range of supercoiled topoisomers) in the supercoiled plasmids (Mu GBS with SPY device) .   
  
 
<!-- -->
 
<!-- -->

Revision as of 22:13, 21 September 2011

Mu/Spy Ligation

A device to detects the level of stress due to a GBS being present on the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 191
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1203