Difference between revisions of "Part:BBa K607028"

Revision as of 22:08, 21 September 2011

PlasB_LasR-DAS_antipBad/araC with 0.3RBS

LasR protein with moderately fast degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 195
Illegal NheI site found at 1094
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1155
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 643
Illegal AgeI site found at 840
Illegal AgeI site found at 1320
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1337

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 __NOTOC__
 <partinfo>BBa_K607028 short</partinfo>
-LasR protein with moderately fast degradation tag under transcriptional control of PlasB promoter and reverse antisense PBad promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.
+LasR protein with moderately fast degradation tag under transcriptional control of PlasB promoter and reverse antisense pBad/araC promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents (in presence of N-(3-oxododecanoyl)homoserine lactone in the media) an auto-inducing loop in which the PlasB promoter drives expression of its own inducer LasR. The running loop can be turned off through activation of the reverse antisense PBad promoter by arabinose.
 <!-- Add more about the biology of this part here