Difference between revisions of "Part:BBa I757010:Experience"
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===Applications of BBa_I757010=== | ===Applications of BBa_I757010=== | ||
| − | Done by iGEM11 Potsdam_Bioware | + | Done by iGEM11 Potsdam_Bioware<br> |
This beta lactamase was used in combination with the TorA signal sequence (BBa_K208005) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.<br> | This beta lactamase was used in combination with the TorA signal sequence (BBa_K208005) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.<br> | ||
When <i>E.coli XL1 blue</i> where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C, or up to 300 µg/ml ampicillin, when incubated over night at 30 °C.<br> | When <i>E.coli XL1 blue</i> where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C, or up to 300 µg/ml ampicillin, when incubated over night at 30 °C.<br> | ||
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===User Reviews=== | ===User Reviews=== | ||
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Revision as of 22:06, 21 September 2011
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Applications of BBa_I757010
Done by iGEM11 Potsdam_Bioware
This beta lactamase was used in combination with the TorA signal sequence (BBa_K208005) as a new protease activity detector device. The BioBrick parts BBa_K627012 and BBa_K627013 consist of the TorA signal sequence followed by a short peptide sequence used as cleavage site for different proteases (TEV (BBa_K627008-BBa_K627010) and 14_3C (BBa_K627011)) and ends with the beta lactamase.
When E.coli XL1 blue where transformed with our construct, the cells were able to survive at ampicillin concentrations up to 400 µg/ml, when incubated on agar plates over night at 37°C, or up to 300 µg/ml ampicillin, when incubated over night at 30 °C.
User Reviews
UNIQd70727fd17d5e9a9-partinfo-00000000-QINU UNIQd70727fd17d5e9a9-partinfo-00000001-QINU The lactamases shows great activity at 37°C and 30°C incubation over night on agar plates. The transformed E.coli cells were able to survive with ampicillin concentrations up to 400 µg/ml at 37°C and up to 300 µg/ml at 30°C.