Difference between revisions of "Part:BBa K607013"

Latest revision as of 21:40, 21 September 2011

Prm_cI-LVA_antiPlasB with 1.0RBS

This part is a member of Lambda cI autoinducing loop family.

cI with LVA-tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 1.0 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))

Figure 1. This autoinducing loop does not exhibit heterogenity when non-induced. Only the population that is not producing GFP is observed. After 4 hours of incubation approximately only 1,03% of the visible cells are fluorescent while 93,91% are not fluorescent. That means that the loop is not induced through a leaky promoter and is a good candidate for an inducible bistable switch.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1123
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 [[Image:lciaulegend.png|frameless]]
-'''Figure 1.''' This autoinducing loop does not exhibit heterogenity when non-induced. Only the population that is not producing GFP is observed. That means that the loop is not induced through a leaky promoter and is a good candidate for an inducible bistable switch.
+'''Figure 1.''' This autoinducing loop does not exhibit heterogenity when non-induced. Only the population that is not producing GFP is observed. After 4 hours of incubation approximately only 1,03% of the visible cells are fluorescent while 93,91% are not fluorescent. That means that the loop is not induced through a leaky promoter and is a good candidate for an inducible bistable switch.
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