Difference between revisions of "Part:BBa K549004"
 
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The biobrick contains the lacI promotor from E. coli fused with the iron dependent regulator fur from Neisseria
 
The biobrick contains the lacI promotor from E. coli fused with the iron dependent regulator fur from Neisseria
 
meningitidis. Fur regulates genes via binding at fur-binding-boxes.  
 
meningitidis. Fur regulates genes via binding at fur-binding-boxes.  
−
 
−
The sequence of fur is missing in the sequence part of this page. fur follows downstream of the RBS with the following sequence:
 
−
ATGGAAAAATTCAACAATATTGCACAACTGAAAGACAGCGGTCTGAAGGTTACCGGCCCG
 
−
CGTTTGAAGATTTTGGATTTGTTCGAGACGCATGCGGAAGAGCATTTGAGTGCGGAAGAT
 
−
GTGTACCGCATTTTGTTGGAAGAGGGTGTGGAAATCGGTGTGGCGACGATTTACCGTGTG
 
−
CTGACCCAGTTTGAGCAGGCGGGCATTTTGCAACGCCATCATTTTGAAACGGGCAAGGCG
 
−
GTTTATGAGTTGGACAAAGGCGACCACCATGACCACATCGTCTGCGTGAAGTGCGGCGAG
 
−
GTAACGGAGTTCCACAATCCCGAAATCGAAGCCCTGCAAGACAAAATCGCGGAAGAAAAC
 
−
GGCTACCGCATCGTCGATCACGCGCTTTATATGTACGGCGTGTGCAGCGACTGTCAGGCC
 
−
AAGGGCAAACGTTAA
 
  
  

Latest revision as of 21:37, 21 September 2011

LacI promotor fused with the iron dependent regulator fur

The biobrick contains the lacI promotor from E. coli fused with the iron dependent regulator fur from Neisseria meningitidis. Fur regulates genes via binding at fur-binding-boxes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 323