Difference between revisions of "Part:BBa K613022"
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This is a TetR mutant that carries the E37A P39K double mutation.
 
This is a TetR mutant that carries the E37A P39K double mutation.
 
Part of the <html> <a href="http://2011.igem.org/Team:EPF-Lausanne/Our_Project/TetR_mutants/muTetRs">EPFL2011 muTetR collection. </a> </html>
 
Part of the <html> <a href="http://2011.igem.org/Team:EPF-Lausanne/Our_Project/TetR_mutants/muTetRs">EPFL2011 muTetR collection. </a> </html>
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It has an altered tet operator sequence recognition compared to the wild-type tetR variant. And prefers the tetO-4C5G operator.
+
 
 +
It has an altered tet operator sequence recognition compared to the wild-type tetR variant. And prefers the tetO-4C5G operator.
  
  

Revision as of 21:24, 21 September 2011

TetR E37A P39K mutant

This is a TetR mutant that carries the E37A P39K double mutation. Part of the EPFL2011 muTetR collection.

It has an altered tet operator sequence recognition compared to the wild-type tetR variant. And prefers the tetO-4C5G operator.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Designed sequence (2 mutations in yellow):

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQKTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA

LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFD

HQGAEPAFLFGLELIICGLEKQLKCESGS*