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| − | ==Protocol==
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| − | Functionality of this part was tested by treating AHL mixtures with strains expressing this part. Liquid cultures were grown overnight, 1 mL aliquots of these cultures were spun down and resuspended in varying concentrations of AHL in PBS (to start the AHL degradation reaction). This was also done with a culture that contains BBa_K546002 (where aiiA is replaced with GFP).
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| − | After a 3h incubation, the supernatant was collected and subjected to a reporter strain: BBa_K546002. 200 µL samples of reporter strain/AHL mixtures were analyzed with a Molecular Devices Spectramax M2 spectrophotometer, detecting GFP. The result is shown below.
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| − | [[Image: AHL_degradation_by_AiiA_reported_by_BBa_K546002correct.png]]
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| − | This part is functional, as AHL concentrations drop after incubation with <I>E. coli</I> transformed with this part. The GFP expression is much higher in the non treated mixtures, namely.
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| − | <I>Team Wageningen_UR 2011</I>
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| | <!-- End of the user review template --> | | <!-- End of the user review template --> |
| | <!-- DON'T DELETE --><partinfo>BBa_K546001 EndReviews</partinfo> | | <!-- DON'T DELETE --><partinfo>BBa_K546001 EndReviews</partinfo> |
Revision as of 21:10, 21 September 2011
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UNIQ62f6bedfcd3b8d30-partinfo-00000000-QINU
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Youri van Nuland
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UNIQ62f6bedfcd3b8d30-partinfo-00000002-QINU
