Difference between revisions of "Part:BBa K638102"

Revision as of 20:52, 21 September 2011

Arabinose inducible Reflectin A2 generator

Produces Reflectin A2 protein on addition of arabinose.

The reflectin gene is under the control of the pBAD promoter BBa_I0500, which is tightly controlled by two factors:

L-arabinose monosaccharide taken up by the cell from the medium, which acts as an inducer.
AraC protein included in the I0500 biobrick, which acts an a repressor.

Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer. Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli].

Usage and Biology

Best used in an E. coli chassis such as the strain [http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter. See our experience of pBAD for some issues with tuning expression levels.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
Illegal AgeI site found at 1308
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 11: / Line 11: @@
 ===Usage and Biology===
-Best used in an ''E. coli'' chassis such as strain [[http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter.
+Best used in an ''E. coli'' chassis such as the strain [http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter.
 See our [[Part:BBa_I0500:Experience | experience of pBAD]] for some issues with tuning expression levels.