Difference between revisions of "Part:BBa K638102"
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Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer. | Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer. | ||
Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli]. | Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli]. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Best used in an ''E. coli'' chassis such as strain | + | Best used in an ''E. coli'' chassis such as strain [[http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter. |
See our [[Part:BBa_I0500:Experience | experience of pBAD]] for some issues with tuning expression levels. | See our [[Part:BBa_I0500:Experience | experience of pBAD]] for some issues with tuning expression levels. | ||
Revision as of 20:51, 21 September 2011
Arabinose inducible Reflectin A2 generator
Produces Reflectin A2 protein on addition of arabinose.
The reflectin gene is under the control of the pBAD promoter BBa_I0500, which is tightly controlled by two factors:
- L-arabinose monosaccharide taken up by the cell from the medium, which acts as an inducer.
- AraC protein included in the I0500 biobrick, which acts an a repressor.
Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer. Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli].
Usage and Biology
Best used in an E. coli chassis such as strain [[http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783], with constitutive expression of an Arabinose transporter. See our experience of pBAD for some issues with tuning expression levels.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1308 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961