Difference between revisions of "Part:BBa K638101"
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<partinfo>BBa_K638101 short</partinfo>
 
<partinfo>BBa_K638101 short</partinfo>
  
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Arabinose inducible Reflectin A1 generator allows for a titratable expression of the protein. The reflectin gene is under the control of the pBAD promoter [[Part:BBa_I0500 I0500]], which is tightly controlled by two factors:
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Arabinose inducible Reflectin A1 generator allows for a titratable expression of the protein. The reflectin gene is under the control of the pBAD promoter [[Part:BBa_I0500 | BBa_I0500]], which is tightly controlled by two factors:
 
*'''L-arabinose''' monosaccharide taken up by the cell from the medium, which acts as an ''inducer''.  
 
*'''L-arabinose''' monosaccharide taken up by the cell from the medium, which acts as an ''inducer''.  
 
*'''AraC''' protein included in the I0500 biobrick, which acts an a ''repressor''.  
 
*'''AraC''' protein included in the I0500 biobrick, which acts an a ''repressor''.  

Revision as of 20:39, 21 September 2011

Arabinose inducible Reflectin A1 generator

Arabinose inducible Reflectin A1 generator allows for a titratable expression of the protein. The reflectin gene is under the control of the pBAD promoter BBa_I0500, which is tightly controlled by two factors:

  • L-arabinose monosaccharide taken up by the cell from the medium, which acts as an inducer.
  • AraC protein included in the I0500 biobrick, which acts an a repressor.

Therefore, the araC-pBAD system offers regulatable control of gene expression in the presence of the inducer and highly repressed in the absence of the inducer. In order to obtain a linear titratable relation between the concentration of arabinose in the medium and the level of reflectin expression, a special strain of bacteria [http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783] needs to be used. Read more about [http://2011.igem.org/Team:Cambridge/Experiments/Low_Level_Expression the pBAD and arabinose system in E.coli].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961