Difference between revisions of "Part:BBa K638102"
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<partinfo>BBa_K638102 short</partinfo>
 
<partinfo>BBa_K638102 short</partinfo>
  
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Arabinose inducible [[Part:BBa_K638002|Reflectin A2]] generator.   
+
Produces [[Part:BBa_K638002|Reflectin A2]] protein on addition of Arabinose.   
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 20:23, 21 September 2011

Arabinose inducible Reflectin A2 generator

Produces Reflectin A2 protein on addition of Arabinose.

Usage and Biology

Best used in an E. coli chassis such as strain Bw27783, with constitutive expression of an Arabinose transporter. See our experience of pBAD for some issues with tuning expression levels.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1308
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961