Difference between revisions of "Part:BBa K525710:Design"

(→‎Design Notes)
(→‎Source)
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PCR on the ''Escherichia coli'' TOP 10 DNA cloned into BioBrick Vectors
 
PCR on the ''Escherichia coli'' TOP 10 DNA cloned into BioBrick Vectors
  
−
     Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix in the rev primer.
+
     Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix and His-tag in the rev primer.
−
         fwd:  
+
         fwd: 5'-acgtgaattcgcggccgcttctagagtaatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaac-3'
−
         rev:
+
         rev: 5'-acgtctgcagcggccgctactagtaatgatgatgatgatgatggctacccagcaaacgc-3'
  
 
===References===
 
===References===

Revision as of 20:21, 21 September 2011

DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1663


Design Notes

This is the complete sequence of NAD+ -dependent DNA ligase with PT7, RBS and His-tag

Source

PCR on the Escherichia coli TOP 10 DNA cloned into BioBrick Vectors 

   Primers with BioBrick prefix, pT7-Promoter and RBS in the fwd primer. Suffix and His-tag in the rev primer.
       fwd: 5'-acgtgaattcgcggccgcttctagagtaatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaac-3'
       rev: 5'-acgtctgcagcggccgctactagtaatgatgatgatgatgatggctacccagcaaacgc-3'

References