Difference between revisions of "Part:BBa K607015"

Revision as of 19:58, 21 September 2011

cI

cI without degradation tag The cI gene codes for the bacteriophage λ repressor(1). This bacteriophage λ-repressor from phage lambda does not contain a ssrA tag. The ssrA tag is a target for proteolytic enzymes and causes degradation of the protein(2). When this gene is transcribed, it will not be degraded that fast by proteolytic enzymes ( because it lacks the ssrA tag) and so the protein half-life will be longer compared to cI with a degradation tag.

References: (1)Robert T. Sauer* and Robert Anderegg. Primary Structure of the λ Repressor. Biochemistry, 1978. (2) Jeff Hasty, David McMillen, Farren Isaacs and James J. Collins. Computational studies of gene regulatory networks: in numero molecular biology. Nature 268

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K607015 short</partinfo>
 cI without degradation tag
+The cI gene codes for the bacteriophage λ repressor(1). This bacteriophage λ-repressor from phage lambda does not contain a ssrA tag. The ssrA tag is a target for proteolytic enzymes and causes degradation of the protein(2). When this gene is transcribed, it will not be degraded that fast by proteolytic enzymes ( because it lacks the ssrA tag) and so the protein half-life will be longer compared to cI with a degradation tag.
+References:
+(1)Robert T. Sauer* and Robert Anderegg. Primary Structure of the λ Repressor. Biochemistry, 1978.
+(2) Jeff Hasty, David McMillen, Farren Isaacs and James J. Collins. Computational studies of gene regulatory networks: in numero molecular biology. Nature 268
 <!-- Add more about the biology of this part here