Difference between revisions of "Part:BBa K638001"

Revision as of 19:57, 21 September 2011

Reflectin A1 from Loligo

This is the coding region for [http://www.ncbi.nlm.nih.gov/nuccore/FJ824804.1 Reflectin A1 protein] (originally found in the mantle of squid Loligo pealeii) codon optimised for E. coli. [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator BBa_K638202 . We also exported it as a TorA fusion.

Usage and Biology

In the squid this protein is believed to self assemble with membranes to form iridophore platelets - stacks of protein ultrastructure evenly spaced to form a Bragg stack. This creates living structural colour from thin film interference effects. In L. pealeii reflectins are phosphorylated, with [http://www.ncbi.nlm.nih.gov/pubmed/19776150 changes in phosphorylation status correlating with changes in tissue colour]. Read more about [http://2011.igem.org/Team:Cambridge/Project/Background the background to this protein].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 22
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K638001 short</partinfo>
-This is the coding region for reflectin protein A1 (originally found in the mantle of squid ''Loligo pealeii'') codon optimised for ''E. coli''.
+This is the coding region for [http://www.ncbi.nlm.nih.gov/nuccore/FJ824804.1 Reflectin A1 protein] (originally found in the mantle of squid ''Loligo pealeii'') codon optimised for ''E. coli''.
 [http://2011.igem.org/Team:Cambridge Cambridge 2011] overexpressed it for purification using the his-tagged generator [[Part:BBa_K638202 | BBa_K638202 ]].  We also exported it as a [[Part:BBa_K638401 | TorA fusion]].