Difference between revisions of "Part:BBa K638001:Experience"
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===Applications of BBa_K638001=== | ===Applications of BBa_K638001=== | ||
| − | When extracted and purified, this protein can be used to make optical devices including thin films and diffraction gratings. | + | When extracted and purified, this protein can be used to make optical devices including thin films and diffraction gratings as has been demonstated by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.] Additionally, Cambridge iGEM Team 2011 further developed the method for making [http://2011.igem.org/Team:Cambridge/Experiments/Thin_Films thin films]. |
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| + | First the protein must be dissolved in an ionic solvent such as [http://en.wikipedia.org/wiki/Hexafluoro-2-propanol HFIP] (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work. A razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a [http://2011.igem.org/Team:Cambridge#/Protocols/Flow_coating flow coater] or a [http://2011.igem.org/Team:Cambridge#/Protocols/Spin_Coating spin coater device]. | ||
| + | |||
| + | <center> | ||
| + | <gallery widths=400px> | ||
| + | Image:cam_thin_film.png | Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]'']] | ||
| + | |||
| + | Image:cam_thin_film_I.png | Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]'' ]] | ||
| + | </gallery> | ||
| + | </center> | ||
===Pictures=== | ===Pictures=== | ||
Revision as of 19:40, 21 September 2011
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Description
Applications of BBa_K638001
When extracted and purified, this protein can be used to make optical devices including thin films and diffraction gratings as has been demonstated by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.] Additionally, Cambridge iGEM Team 2011 further developed the method for making [http://2011.igem.org/Team:Cambridge/Experiments/Thin_Films thin films].
First the protein must be dissolved in an ionic solvent such as [http://en.wikipedia.org/wiki/Hexafluoro-2-propanol HFIP] (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work. A razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a [http://2011.igem.org/Team:Cambridge#/Protocols/Flow_coating flow coater] or a [http://2011.igem.org/Team:Cambridge#/Protocols/Spin_Coating spin coater device].
Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.]]]
Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.] ]]
Pictures
Compatibility
Has been shown to work with plasmids:
- pSB1A3
- pSB3K3
- pSB1C3
On high copy-plasmids such as pSB1A3, this protein has been shown to go into inclusion bodies.
User Reviews
UNIQa5e44599a429a60b-partinfo-00000001-QINU UNIQa5e44599a429a60b-partinfo-00000002-QINU