Difference between revisions of "Part:BBa K660603"
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This is a composite part featuring the OmpF promoter and Weak RBS.<br/><br/> | This is a composite part featuring the OmpF promoter and Weak RBS.<br/><br/> | ||
| − | It is part of the 2011 University of Glasgow Promoter/RBS library.<br/> | + | It is part of the 2011 University of Glasgow Promoter/RBS library.<br/> |
| + | |||
| + | <u>Design</u><br/><br/> | ||
| + | The small size of the ribosome binding site means that it cannot be visualised on a gel. To solve this problem, we used AlwnI restriction enzyme in a method that is detailed on our wiki. <br/> | ||
| + | |||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 19:33, 21 September 2011
OmpF + W.RBS
This is a composite part featuring the OmpF promoter and Weak RBS.
It is part of the 2011 University of Glasgow Promoter/RBS library.
Design
The small size of the ribosome binding site means that it cannot be visualised on a gel. To solve this problem, we used AlwnI restriction enzyme in a method that is detailed on our wiki.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]