Difference between revisions of "Part:BBa K638202"
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<partinfo>BBa_K638202 short</partinfo>
 
<partinfo>BBa_K638202 short</partinfo>
  
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Arabinose inducible Poly-His Reflectin A1 generator produces a poly-histidine (his-tagged) form of Reflectin A1([[Part:BBa_K638001 | K638001]]). Bacteria expressing this construct will produce Reflectin A1 with a poly-histidine tag at the N-terminus in order to enable purification with a his-affinity column used in conjunction with nickel or cobalt resins.  
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Arabinose inducible Poly-His Reflectin A1 generator produces a poly-histidine (his-tagged) form of Reflectin A1([[Part:BBa_K638001 | BBa_K638001]]).  
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Bacteria expressing this construct will produce Reflectin A1 with a poly-histidine tag at the N-terminus in order to enable purification with a his-affinity column used in conjunction with nickel or cobalt resins.  
  
 
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Revision as of 19:19, 21 September 2011

Arabinose inducible Poly-His Reflectin A1 generator

Arabinose inducible Poly-His Reflectin A1 generator produces a poly-histidine (his-tagged) form of Reflectin A1( BBa_K638001). Bacteria expressing this construct will produce Reflectin A1 with a poly-histidine tag at the N-terminus in order to enable purification with a his-affinity column used in conjunction with nickel or cobalt resins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1261
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961