Difference between revisions of "Part:BBa K525008"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K525008 short</partinfo>
 
<partinfo>BBa_K525008 short</partinfo>
  
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PT7 + RBS + SbpA || BisdA || BisdB
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Fusion protein of SbpA || BisdA || BisdB (RFC 25) for S-layer immobilized, cell-free BPA degradation after expression in ''E. coli'' using T7 promoter when combined with a ferredoxin-NADP<sup>+</sup> oxidoreductase.
  
  

Latest revision as of 19:18, 21 September 2011

Fusion protein of S-Layer SbpA with T7/lac and BisdA and BisdB

Fusion protein of SbpA || BisdA || BisdB (RFC 25) for S-layer immobilized, cell-free BPA degradation after expression in E. coli using T7 promoter when combined with a ferredoxin-NADP+ oxidoreductase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 221
    Illegal BamHI site found at 3792
    Illegal BamHI site found at 4530
    Illegal XhoI site found at 1996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 4783
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 493
    Illegal BsaI.rc site found at 622