Difference between revisions of "Part:BBa K638202:Experience"
(→Applications of BBa_K638202) |
(→Applications of BBa_K638202) |
||
| Line 7: | Line 7: | ||
The original method for making reflectin thin films was developed by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.] | The original method for making reflectin thin films was developed by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.] | ||
| − | <gallery widths= | + | <center> |
| + | <gallery widths=500px> | ||
Image:cam_thin_film.png | Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]'']] | Image:cam_thin_film.png | Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]'']] | ||
Image:cam_thin_film_I.png | Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]'' ]] | Image:cam_thin_film_I.png | Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. ''Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer ''et al'', 2007.]'' ]] | ||
</gallery> | </gallery> | ||
| + | </center> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:14, 21 September 2011
Applications of BBa_K638202
The purified his-tagged Reflectin A1 has optical properties, best viewed when made into a thin film. First the protein must be dissolved in an ionic solvent such as HFIP (hazardous - use in a fume hood) and then can be coated onto a highly clean smooth substrate. Silicon Wafers and glass microscope slides have been shown to work, a razorblade can be used to scrape across a single drop to form a thin film, but more uniform results can be achieved with the use of a flow coater or a spin coater device.
The original method for making reflectin thin films was developed by [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.]
Fig.1 Schematic diagram of the flow-coating technique for casting thin films of reflectin protein onto a silicon-wafer substrate. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.]]]
Fig.2 Reflectin films exhibiting uniform thicknesses cast from a 15% w/w (left) and 10% w/w (right) reflectin/HFIP solution. Scale bar: 2 cm. Image taken from [http://www.nature.com/nmat/journal/v6/n7/full/nmat1930.html Kramer et al, 2007.] ]]
User Reviews
UNIQa1acf095b6a9bd5a-partinfo-00000001-QINU UNIQa1acf095b6a9bd5a-partinfo-00000002-QINU