Difference between revisions of "Part:BBa K546003"

Revision as of 19:13, 21 September 2011

Lux pL controlled LuxR + lux pR promoter.

This part was made as a replacement for a defective version of BBa_F2621. After sequencing we noticed that BBa_F2621 was lacking the BBa_R0063 promoter. We tested the expression levels of BBa_K546003 and BBa_F2621 using RBS-J04031-B0015 as a reporter (See BBA_K546002). BBa_K546003 had a significantly higher GFP signal than the F2621 BioBrick part we received, this indicated that we fixed it.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1101

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K546003 short</partinfo>
-This part was made as a replacement for a defective version of BBa_F2621 which lacked the R0063 promoter, but was otherwise intact and functional. It shows a significantly higher response to 3OC6HSL than the incomplete BBa_F2621 (which is present in the Registry as BBa_K317037).
+This part was made as a replacement for a defective version of BBa_F2621. After sequencing we noticed that BBa_F2621 was lacking the BBa_R0063 promoter. We tested the expression levels of BBa_K546003 and BBa_F2621 using RBS-J04031-B0015 as a reporter (See BBA_K546002). BBa_K546003 had a significantly higher GFP signal than the F2621 BioBrick part we received, this indicated that we fixed it.
 <!-- Add more about the biology of this part here