Difference between revisions of "Part:BBa K546000"

Revision as of 19:00, 21 September 2011

Lux pL controlled LuxR with lux pR autoinducing LuxI(lva tag)- AHL.

This device has two protein generators: lux pl-RBS-C0062-B0015 produces LuxR. LuxR forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreasing the transcriptional rate of the lux pL promoter.

The quorum sensing molecule AHL is produced by LuxI which is under the control of the lux pR promoter. In combination with LuxR, AHL thus increases the production of AHL, a positive feedback loop.

Safety Aspects

There's a small note regarding the biosafety of this BioBrick composite part. Upon release into the environment (this includes infection of a laboratory worker) this BioBrick composite part might alter the pathogenicity of pathogens that rely on quorum sensing to infect hosts. Pathogenic species that might be involved can be found on the following external [http://2011.igem.org/Team:Wageningen_UR/Safety/One#Risk_Identification_of_BioBrick_System_Inside_the_Cell_Chassis biological safety page].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1821
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1101

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 <partinfo>BBa_K546000 short</partinfo>
-The F2621 BioBrick device contains a -lux pL regulated- luxR repressor/activator gene and a -lux pR regulated- 3OC6HSL synthetase, an AHL [http://en.wikipedia.org/wiki/Autoinducer autoinducer molecule] producing enzyme. The lux pL promoter yields a weak constitutive downstream expression. When LuxR and 3OC6HSL are produced, the lux pL promoter, however, will be repressed by a complex of the two. Complementary to the lux pL promoter, the lux pR promoter with a complex of luxR and  3OC6HSL will enable enlarged activation of the downstream synthetase gene, thus increase the concentration 3OC6HSL.
+This device has two protein generators:
+lux pl-RBS-C0062-B0015 produces LuxR. LuxR forms a complex with the quorum sensing molecule AHL and this complex subsequently increases the transcriptional rate of the lux pR promoter while also decreasing the transcriptional rate of the lux pL promoter.
+The quorum sensing molecule AHL is produced by LuxI which is under the control of the lux pR promoter. In combination with LuxR, AHL thus increases the production of AHL, a positive feedback loop.
 <!-- Add more about the biology of this part here