Difference between revisions of "Part:BBa K613022"
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K613022 short</partinfo>
 
<partinfo>BBa_K613022 short</partinfo>
  
−
This is a TetR mutant with the E37AP39K double mutation. It has an altered tet operator sequence recognition compared to the wild-type tetR variant.  
+
This is a TetR mutant that carries the E37AP39K double mutation. It has an altered tet operator sequence recognition compared to the wild-type tetR variant. And prefers the tetO-4C5G operator.
−
 
+
  
−
== Characterization ==
 
  
−
The mutant was characterized in vitro by
 
  
  

Revision as of 18:37, 21 September 2011

TetR E37A P39K mutant

This is a TetR mutant that carries the E37AP39K double mutation. It has an altered tet operator sequence recognition compared to the wild-type tetR variant. And prefers the tetO-4C5G operator.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Designed sequence (2 mutations in yellow):

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQKTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA

LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFD

HQGAEPAFLFGLELIICGLEKQLKCESGS*