Difference between revisions of "Part:BBa K613022"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K613022 short</partinfo>
 
<partinfo>BBa_K613022 short</partinfo>
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This is a TetR mutant with the E37AP39K double mutation. It has an altered tet operator sequence recognition compared to the wild-type tetR variant.  
 
This is a TetR mutant with the E37AP39K double mutation. It has an altered tet operator sequence recognition compared to the wild-type tetR variant.  
  
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'''Characterization'''
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== Characterization ==
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The mutant was characterized in vitro by  
 
The mutant was characterized in vitro by  
  
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and in vivo 
 
  
 
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Revision as of 18:36, 21 September 2011


TetR E37A P39K mutant

This is a TetR mutant with the E37AP39K double mutation. It has an altered tet operator sequence recognition compared to the wild-type tetR variant.


Characterization

The mutant was characterized in vitro by


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Designed sequence (2 mutations in yellow):

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQKTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCA

LLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFD

HQGAEPAFLFGLELIICGLEKQLKCESGS*