Difference between revisions of "Part:BBa K549004"

 
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__NOTOC__
 
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<partinfo>BBa_K549004 short</partinfo>
 
<partinfo>BBa_K549004 short</partinfo>
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The biobrick contains the lacI promotor from E. coli fused with the iron dependent regulator fur from Neisseria
 
The biobrick contains the lacI promotor from E. coli fused with the iron dependent regulator fur from Neisseria
 
meningitidis. Fur regulates genes via binding at fur-binding-boxes.  
 
meningitidis. Fur regulates genes via binding at fur-binding-boxes.  
 +
 +
The sequence of fur is missing in the sequence part of this page. fur follows downstream of the RBS with the following sequence:
 +
ATGGAAAAATTCAACAATATTGCACAACTGAAAGACAGCGGTCTGAAGGTTACCGGCCCG
 +
CGTTTGAAGATTTTGGATTTGTTCGAGACGCATGCGGAAGAGCATTTGAGTGCGGAAGAT
 +
GTGTACCGCATTTTGTTGGAAGAGGGTGTGGAAATCGGTGTGGCGACGATTTACCGTGTG
 +
CTGACCCAGTTTGAGCAGGCGGGCATTTTGCAACGCCATCATTTTGAAACGGGCAAGGCG
 +
GTTTATGAGTTGGACAAAGGCGACCACCATGACCACATCGTCTGCGTGAAGTGCGGCGAG
 +
GTAACGGAGTTCCACAATCCCGAAATCGAAGCCCTGCAAGACAAAATCGCGGAAGAAAAC
 +
GGCTACCGCATCGTCGATCACGCGCTTTATATGTACGGCGTGTGCAGCGACTGTCAGGCC
 +
AAGGGCAAACGTTAA
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:17, 21 September 2011

LacI promotor fused with the iron dependent regulator fur

The biobrick contains the lacI promotor from E. coli fused with the iron dependent regulator fur from Neisseria meningitidis. Fur regulates genes via binding at fur-binding-boxes.

The sequence of fur is missing in the sequence part of this page. fur follows downstream of the RBS with the following sequence: ATGGAAAAATTCAACAATATTGCACAACTGAAAGACAGCGGTCTGAAGGTTACCGGCCCG CGTTTGAAGATTTTGGATTTGTTCGAGACGCATGCGGAAGAGCATTTGAGTGCGGAAGAT GTGTACCGCATTTTGTTGGAAGAGGGTGTGGAAATCGGTGTGGCGACGATTTACCGTGTG CTGACCCAGTTTGAGCAGGCGGGCATTTTGCAACGCCATCATTTTGAAACGGGCAAGGCG GTTTATGAGTTGGACAAAGGCGACCACCATGACCACATCGTCTGCGTGAAGTGCGGCGAG GTAACGGAGTTCCACAATCCCGAAATCGAAGCCCTGCAAGACAAAATCGCGGAAGAAAAC GGCTACCGCATCGTCGATCACGCGCTTTATATGTACGGCGTGTGCAGCGACTGTCAGGCC AAGGGCAAACGTTAA


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 323