Difference between revisions of "Part:BBa K613022"

Revision as of 17:57, 21 September 2011

TetR E37A P39K mutant

This is a TetR mutant with the EA37PK39 double mutation

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Designed sequence (2 mutations in yellow):

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQKTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAK</p><p class=MsoNormal>VHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*</p>

@@ Line 15: / Line 15: @@
 normal'><span lang=EN-GB style='font-size:14.0pt;mso-bidi-font-size:10.0pt;
 font-family:"Courier New";mso-bidi-font-family:"Times New Roman";mso-ansi-language:
-EN-GB'>MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGV<span style='background:yellow'>A</span>Q<span style='background:yellow'>K</span>TLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAK</p><p>VHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*</span></b></span></span><b
+EN-GB'>MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGV<span style='background:yellow'>A</span>Q<span style='background:yellow'>K</span>TLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAK</span></b></p><p class=MsoNormal><span class=SpellE><span class=GramE><b style='mso-bidi-font-weight:
+normal'><span lang=EN-GB style='font-size:14.0pt;mso-bidi-font-size:10.0pt;
+font-family:"Courier New";mso-bidi-font-family:"Times New Roman";mso-ansi-language:
+EN-GB'>VHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*</span></b></span></span><b
 style='mso-bidi-font-weight:normal'><span lang=EN-GB style='font-size:14.0pt;
 mso-bidi-font-size:10.0pt;font-family:"Courier New";mso-bidi-font-family:"Times New Roman";