Difference between revisions of "Part:BBa K638401"

Revision as of 17:34, 21 September 2011

Arabinose inducible TorA Export Tagged Reflectin A1 Poly His generator

This construct is a Reflectin A1 protein with a TorA signal peptide fused to the N terminus, which has been shown to allow export of GFP via the twin arginine pathway (see information on Part:BBa_K638402). In addition a poly his tag has been added to the C terminus and a pBAD promoter will control expression of the fusion protein on induction with arabinose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
Illegal BamHI site found at 1369
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

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 __NOTOC__
 <partinfo>BBa_K638401 short</partinfo>
-This construct is a Reflectin A1 protein with a TorA signal peptide on the N terminus, which has been shown to allow export of GFP via the twin arginine pathway. In addition a poly his tag has been added to the C terminus and a pBAD promoter will control expression of the fusion protein on induction with arabinose.
+This construct is a Reflectin A1 protein with a [[Part:BBa_K638402|TorA signal peptide]] fused to the N terminus, which has been shown to allow export of GFP via the twin arginine pathway (see information on [[Part:BBa_K638402]]). In addition a poly his tag has been added to the C terminus and a pBAD promoter will control expression of the fusion protein on induction with arabinose.
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