Difference between revisions of "Part:BBa K592200:Design"
| Line 10: | Line 10: | ||
===Source=== | ===Source=== | ||
| − | + | The P22 late terminator come from the genome of the Salmonella phage P22 (GenBank: AF217253.1, 19662..19702). | |
===References=== | ===References=== | ||
Datsenko, K. A. and B. L. Wanner (2000). "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products." Proc Natl Acad Sci U S A 97(12): 6640-5. | Datsenko, K. A. and B. L. Wanner (2000). "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products." Proc Natl Acad Sci U S A 97(12): 6640-5. | ||
Revision as of 17:10, 21 September 2011
Low to medium copy BioBrick standard vector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3116
Illegal NheI site found at 1403
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3122 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3116 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3116
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3116
Plasmid lacks a suffix.
Illegal XbaI site found at 3131
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 1969
Illegal AgeI site found at 994
Illegal AgeI site found at 1317 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 289
Design Notes
To make the plasmid usable for Lambda Red recombineering (Datsenko and Wanner, 2000), all sequences with homologies to the E coli chromosome had to be removed. To accomplish this, the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
Source
The P22 late terminator come from the genome of the Salmonella phage P22 (GenBank: AF217253.1, 19662..19702).
References
Datsenko, K. A. and B. L. Wanner (2000). "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products." Proc Natl Acad Sci U S A 97(12): 6640-5.