Difference between revisions of "Part:BBa K607020"
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<partinfo>BBa_K607020 short</partinfo> | <partinfo>BBa_K607020 short</partinfo> | ||
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| + | This part is a member of [https://parts.igem.org/Lambda_cI_autoinducing_loop_family Lambda cI autoinducing loop family]. | ||
cI without degradation tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI)) | cI without degradation tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI)) | ||
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| + | [[Image:lciau8.png|Caption test]] | ||
| + | [[Image:lciaulegend.png|frameless]] | ||
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| + | '''Figure 1.''' This autoinducing loop does exhibit heterogenity when non-induced. Two populations are visible - a population that is not producing GFP and a population that is producing GFP is observed. That means that the loop is induced in some of the cells through a leaky promoter. Changing the promoter to a less leaky version might make it a good candidate for an inducible bistable switch. | ||
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Latest revision as of 17:07, 21 September 2011
Prm_cI_antiPlasB with 0.3RBS
This part is a member of Lambda cI autoinducing loop family.
cI without degradation tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.3 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))
Figure 1. This autoinducing loop does exhibit heterogenity when non-induced. Two populations are visible - a population that is not producing GFP and a population that is producing GFP is observed. That means that the loop is induced in some of the cells through a leaky promoter. Changing the promoter to a less leaky version might make it a good candidate for an inducible bistable switch.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1060
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]