Difference between revisions of "Part:BBa K525404:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K525404 short</partinfo>
 
<partinfo>BBa_K525404 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
Fusion Protein of BBa_K525403 and BBa_J18932
 
  
 +
* <partinfo>K525403</partinfo> fused to <partinfo>J18931</partinfo>
 +
** <partinfo>K525403</partinfo> synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
 +
 +
* NgoMIV restriction site downstream the start codon to easily fuse an N-terminal domain to the coding sequence
 +
 +
* AgeI restriction site upstream of stop codon to easily fuse a C-terminal domain to the coding sequence
  
  
 
===Source===
 
===Source===
  
−
Fusion Protein of S-Layer sbpA and mCherry RFP
+
* <partinfo>K525403</partinfo> fused to <partinfo>J18931</partinfo>
 +
** <partinfo>K525403</partinfo> synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
 +
 
 +
* Promoter: fusion promoter between T7 promoter and lac-operator
 +
** T7 promoter from T7 phage
 +
** lac-operator from ''E. coli''
 +
 
 +
* <partinfo>K525401</partinfo> S-layer gene ''sbpA'' synthesized, originated in ''Lysinbacillus sphaericus'' CCM 2177
 +
 
 +
* <partinfo>J18932</partinfo> red fluorescent protein (RFP) mCherry
 +
** from parts.igem
 +
** was synthesized before it was sent in
 +
 
  
 
===References===
 
===References===
 +
Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of ''Lysinibacillus sphaericus'' CCM 2177 for the Generation of Functionalized Nanoarrays, ''Bioconjugate Chem'' [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903].

Latest revision as of 17:06, 21 September 2011

Fusion Protein of S-Layer SbpA and mCherry RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 104
    Illegal BglII site found at 221
    Illegal XhoI site found at 1996
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 76
    Illegal AgeI site found at 3904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 493
    Illegal BsaI.rc site found at 622


Design Notes

  • NgoMIV restriction site downstream the start codon to easily fuse an N-terminal domain to the coding sequence
  • AgeI restriction site upstream of stop codon to easily fuse a C-terminal domain to the coding sequence


Source

  • Promoter: fusion promoter between T7 promoter and lac-operator
    • T7 promoter from T7 phage
    • lac-operator from E. coli
  • BBa_K525401 S-layer gene sbpA synthesized, originated in Lysinbacillus sphaericus CCM 2177
  • BBa_J18932 red fluorescent protein (RFP) mCherry
    • from parts.igem
    • was synthesized before it was sent in


References

Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of Lysinibacillus sphaericus CCM 2177 for the Generation of Functionalized Nanoarrays, Bioconjugate Chem [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903].