Difference between revisions of "Part:BBa K515002"

Revision as of 16:39, 21 September 2011

PA2652

Description

Part of BioBrick BBa_K515102, includes optimised insulator, RBS and coding sequence for a malate-responsive chemoreceptor PA2652 from Pseudomonas aeruginosa PA01. Th insulator allows the strength of the RBS to remain the same even when the promoters for this BioBrick are changed using PCR, with the insulator as the start for primers. The translation initiation rate for the RBS sequence is 42010.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 675
Illegal NgoMIV site found at 777
Illegal AgeI site found at 83
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 984

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 <p><b>Description</b></p>
-<p>Part of biobrick <a href="https://parts.igem.org/Part:BBa_K515102"> BBa_K515102</a>, includes optimised insulator, RBS and coding sequence for malate responsive chemoreceptor PA2652 from <i>Pseudomonas aeruginosa PA01</i>. Insulator allows the strength of the RBS to remain the same even when the promoters for this biobrick are changed using PCR, with insulator as start for primers.The translation initiation rate for the RBS sequence is 42010.</p>
+<p>Part of BioBrick <a href="https://parts.igem.org/Part:BBa_K515102"> BBa_K515102</a>, includes optimised insulator, RBS and coding sequence for a malate-responsive chemoreceptor PA2652 from <i>Pseudomonas aeruginosa PA01</i>. Th insulator allows the strength of the RBS to remain the same even when the promoters for this BioBrick are changed using PCR, with the insulator as the start for primers. The translation initiation rate for the RBS sequence is 42010.</p>
 </html>
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