Difference between revisions of "Part:BBa K627012:Design"
UP Sebastian (Talk | contribs) (→Design Notes) |
UP Sebastian (Talk | contribs) (→Design Notes) |
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===Design Notes=== | ===Design Notes=== | ||
| − | This biobrick was built by PCR using the following PCR primers: | + | This biobrick was built by PCR using the following PCR primers:<br> |
| − | p_TorA-f: | + | * p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC |
| − | CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC | + | * o_Bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC |
| − | + | ||
| − | CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC | + | |
===Source=== | ===Source=== | ||
Revision as of 16:33, 21 September 2011
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 117
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 143
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 796
Design Notes
This biobrick was built by PCR using the following PCR primers:
- p_TorA-f: CTTCTAGATGAACAATAACGATCTCTTTCAGGCATC
- o_Bla_igem_r: CTACTAGTATTAACCGGTCCAATGCTTAATCAGTGAGGCAC