Difference between revisions of "Part:BBa K592202"
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<partinfo>BBa_K592202 short</partinfo> | <partinfo>BBa_K592202 short</partinfo> | ||
BBa_K592202 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and a kanamycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the kanamycin resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase. | BBa_K592202 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and a kanamycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the kanamycin resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase. | ||
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| + | [[Image:K592202-J04450.png]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | ===References=== | ||
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| + | Datsenko, K. A. and B. L. Wanner (2000). "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products." Proc Natl Acad Sci U S A 97(12): 6640-5. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K592202 SequenceAndFeatures</partinfo> | <partinfo>BBa_K592202 SequenceAndFeatures</partinfo> | ||
Revision as of 16:25, 21 September 2011
Low to medium copy Lambda Red recombineering compatible plasmid
BBa_K592202 is a BioBrick friendly vector with low to medium copy p15A replication origin (BBa_I50032) and a kanamycin antibiotic resistance marker, usable for Lambda Red recombineering in E coli. The E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22, and the kanamycin resistance marker is flanked by FRT sites so that the resistance marker can be removed after chromosomal integration using the FLP recombinase.
References
Datsenko, K. A. and B. L. Wanner (2000). "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products." Proc Natl Acad Sci U S A 97(12): 6640-5.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1806
Illegal XbaI site found at 3199
Illegal PstI site found at 2629 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3409
Illegal NheI site found at 1403
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal PstI site found at 2629
Illegal NotI site found at 9
Illegal NotI site found at 3415 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3409
Illegal BglII site found at 2842 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3409
Illegal suffix found at 2
Illegal XbaI site found at 1806
Illegal XbaI site found at 3199
Illegal PstI site found at 2629 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3409
Plasmid lacks a suffix.
Illegal XbaI site found at 1806
Illegal XbaI site found at 3199
Illegal XbaI site found at 3424
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal PstI site found at 2629
Illegal NgoMIV site found at 1895
Illegal NgoMIV site found at 2178
Illegal AgeI site found at 994
Illegal AgeI site found at 1317 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 289
Illegal SapI site found at 2118
Illegal SapI site found at 2328