Difference between revisions of "Part:BBa K525412:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K525412 short</partinfo> | <partinfo>BBa_K525412 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | * <partinfo>K525403</partinfo> fused to <partinfo>K525499</partinfo> | ||
| + | ** <partinfo>K525403</partinfo> synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins | ||
| + | ** <partinfo>K525499</partinfo> from ''Escherichia coli'' TOP10 and in Freiburg assembly standard (RFC 25) to easily create fusion proteins | ||
| + | |||
| + | * NgoMIV restriction site downstream the start codon to easily fuse an N-terminal domain to the coding sequence | ||
| + | |||
| + | * AgeI restriction site upstream of stop codon to easily fuse a C-terminal domain to the coding sequence | ||
===Source=== | ===Source=== | ||
| − | + | * <partinfo>K525403</partinfo> fused to <partinfo>K525499</partinfo> | |
| + | ** <partinfo>K525403</partinfo> synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins | ||
| + | |||
| + | * Promoter: fusion promoter between T7 promoter and lac-operator | ||
| + | ** T7 promoter from T7 phage | ||
| + | ** lac-operator from ''E. coli'' | ||
| + | |||
| + | * <partinfo>K525401</partinfo> S-layer gene ''sbpA'' synthesized, originated in ''Lysinbacillus sphaericus'' CCM 2177 | ||
| + | |||
| + | * <partinfo>K525499</partinfo> Ferredoxin-NADP<sup>+</sup> oxidoreductase from ''E. coli'' TOP10 | ||
| + | |||
| + | |||
===References=== | ===References=== | ||
| + | Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of ''Lysinibacillus sphaericus'' CCM 2177 for the Generation of Functionalized Nanoarrays, ''Bioconjugate Chem'' [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903]. | ||
Latest revision as of 16:20, 21 September 2011
Fusion Protein of S-Layer SbpA and NADP+ Oxidoreductase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 104
Illegal BglII site found at 221
Illegal XhoI site found at 1996 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 76
Illegal AgeI site found at 3940 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 493
Illegal BsaI.rc site found at 622
Illegal BsaI.rc site found at 3874
Design Notes
- BBa_K525403 fused to BBa_K525499
- BBa_K525403 synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
- BBa_K525499 from Escherichia coli TOP10 and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
- NgoMIV restriction site downstream the start codon to easily fuse an N-terminal domain to the coding sequence
- AgeI restriction site upstream of stop codon to easily fuse a C-terminal domain to the coding sequence
Source
- BBa_K525403 fused to BBa_K525499
- BBa_K525403 synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
- Promoter: fusion promoter between T7 promoter and lac-operator
- T7 promoter from T7 phage
- lac-operator from E. coli
- BBa_K525401 S-layer gene sbpA synthesized, originated in Lysinbacillus sphaericus CCM 2177
- BBa_K525499 Ferredoxin-NADP+ oxidoreductase from E. coli TOP10
References
Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of Lysinibacillus sphaericus CCM 2177 for the Generation of Functionalized Nanoarrays, Bioconjugate Chem [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903].